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General Description
Human Peripheral Blood Mononuclear Cells (PBMCs) are a heterogeneous population of blood cells with a round nucleus, including lymphocytes, monocytes, and dendritic cells. PBMCs are isolated from peripheral blood and purified using density gradient centrifugation, a process that separates cells based on their density.
Technical Description
Density gradient centrifugation is a technique that uses a liquid gradient with different densities to separate cells. PBMCs have a lower density than other blood cells, such as red blood cells and granulocytes, so they settle at the interface between two layers of the gradient. The cells can then be collected and purified from the other cells.
PBMCs are typically cryopreserved for storage and transportation. Cryopreservation is a process of freezing cells to very low temperatures (-135°C or below) in order to preserve them for long periods of time. Cryopreserved PBMCs can be thawed and used in experiments or clinical applications as needed.
Product Use Examples
Human PBMCs are a valuable resource for research and clinical applications. They provide a concentrated source of immune cells that can be used to study the immune system, develop new treatments, and perform cellular therapy.
Excellos 360 employs Excellos’s deep cell characterization approach to goes deeper than typical cell and donor screening to create a comprehensive immune cell profile for each donor, as well as an assessment of the metabolic and effector potential of their cells that looks beyond surface markers. Learn More about Excellos 360 Here
