Human CD8+ T cells are a type of white blood cell that plays a critical role in the adaptive immune system. They are responsible for killing infected cells and tumor cells. These cells can be isolated from peripheral blood using either positive selection or negative selection.
Positive selection involves using antibodies that bind to CD8 on T cells to capture them on a magnetic bead or column. The beads or column are then washed to remove any other cells, and the selected T cells are released.
Negative selection involves using antibodies that bind to cells without CD8 to capture them on a magnetic bead or column. The beads or column are then washed to any other cells, and the CD8+ T cells are left behind.
Both positive selection and negative selection can be used to isolate high-purity CD8+ T cells. However, positive selection is generally faster and more efficient, while negative selection is less likely to activate the T cells.
Product Use Examples
These cells are used in a variety of research and clinical applications, including: immunology research, cellular therapy and vaccine development.