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General Description
Human CD3+ T cells are a type of white blood cell that plays a central role in the adaptive immune system. They are responsible for recognizing and responding to specific antigens, such as those from viruses, bacteria, and tumor cells. CD3+ T cells can be isolated from peripheral blood using either positive selection or negative selection.
Technical Description
CD3 is a surface protein that is expressed on all T cells. It is part of the T cell receptor (TCR) complex, which is responsible for recognizing antigens. CD3 also plays a role in T cell activation and signaling.
Positive selection involves using antibodies that bind to CD3 to capture T cells on a magnetic bead or column. The beads or column are then washed to remove any other cells, and the T cells are released.
Negative selection involves using antibodies that bind to non-T cells to capture them on a magnetic bead or column. The beads or column are then washed to remove the non-T cells, and the T cells are left behind.
Both positive selection and negative selection can be used to isolate high-purity CD3+ T cells. However, positive selection is generally faster and more efficient, while negative selection is less likely to activate the T cells.
Product Use Examples
Human CD3+ T cells are used in a variety of research and clinical applications, including: immunology research, cellular therapy and vaccine development.
Excellos 360 employs Excellos’s deep cell characterization approach to goes deeper than typical cell and donor screening to create a comprehensive immune cell profile for each donor, as well as an assessment of the metabolic and effector potential of their cells that looks beyond surface markers. Learn More about Excellos 360 Here
